Trung tâm Thông tin - Thư viện Trường Đại học Y Dược Hải Phòng

NLM	C14
Tác giả CN	Trương Thị Tuyết Anh - Cb.
Nhan đề	Research on the culture process and establishment of immortal cell lines from peripheral blood of newly diagnosed acute leukemia patients
Thông tin xuất bản	H. :Bộ Khoa học và Công nghệ,2025
Tóm tắt	The immortalization culture protocol was successfully established using two media: RPMI + 20% FBS and RPMI + 5% FHS. The immortal cell lines from these two media showed a cell doubling time of 48–72 hours. In the StemMACS matrix gel, all cell lines differentiated, while in the 10% FBS medium, two out of three cell lines died after two weeks of culture. The immortalized cells maintained their typical blast morphology and were able to proliferate stably for over 30 passages.
Thuật ngữ chủ đề	Hermatology
Từ khóa tự do	Acute leukemia; Blast cells
Tác giả(bs) CN	Nguyễn Thị Thùy Linh
Địa chỉ	100Kho Bài trích(1): BT13538
Tệp tin điện tử	https://vjol.info.vn/JournalofHealthSciences-yduochp/article/view/120721/100393
Tệp tin điện tử	https://lib.hpmu.edu.vn/kiposdata2/tapchi2026/anhbiatc/biakhsk_thumbimage.jpg

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Tag	Giá trị
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004	CD8D34F2-5FD2-4F6D-848F-B5F9FCC89471
005	202605201631
008	260520s2025 vm eng
009	1 0
039	[ ] \|y 20260520163116 \|z phuongntt
084	[ ] \|a C14
100	[ ] \|a Trương Thị Tuyết Anh \|e Cb.
245	[ ] \|a Research on the culture process and establishment of immortal cell lines from peripheral blood of newly diagnosed acute leukemia patients
260	[ ] \|a H. : \|b Bộ Khoa học và Công nghệ, \|c 2025
520	[ ] \|a The immortalization culture protocol was successfully established using two media: RPMI + 20% FBS and RPMI + 5% FHS. The immortal cell lines from these two media showed a cell doubling time of 48–72 hours. In the StemMACS matrix gel, all cell lines differentiated, while in the 10% FBS medium, two out of three cell lines died after two weeks of culture. The immortalized cells maintained their typical blast morphology and were able to proliferate stably for over 30 passages.
650	[ ] \|a Hermatology
653	[ ] \|a Acute leukemia
653	[ ] \|a Blast cells
700	[ ] \|a Nguyễn Thị Thùy Linh
773	[ ] \|t Khoa học sức khỏe
852	[ ] \|a 100 \|b Kho Bài trích \|j (1): BT13538
856	[ ] \|u https://vjol.info.vn/JournalofHealthSciences-yduochp/article/view/120721/100393
856	[1 ] \|u https://lib.hpmu.edu.vn/kiposdata2/tapchi2026/anhbiatc/biakhsk_thumbimage.jpg
890	[ ] \|a 1 \|b 0 \|c 0 \|d 0

Dòng	Mã vạch	Bản sao	Nơi lưu	Tình trạng	Cho phép yêu cầu
1	BT13538	1	Kho Bài trích
#1 BT13538 Nơi lưu Kho Bài trích Tình trạng

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